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Human bronchial epithelial cell line hbe1 immortalized human bronchial epithelial cell line hbe1 derived from a non cf individual.
Human lung epithelial cell line. Bronchial tracheal epithelial cells atcc offers an assortment of products for the culture of airway epithelial cells. A549 cells are adenocarcinomic human alveolar basal epithelial cells and constitute a cell line that was first developed in 1972 by d. Respiratory epithelium or airway epithelium is a type of ciliated columnar epithelium found lining most of the respiratory tract as respiratory mucosa where it serves to moisten and protect the airways. Lung cancer and normal cell lines.
The cells have been derived from the surface epithelium of normal human bronchial tracheal tissue or from the surface epithelium of normal human bronchioles. Some researcher use human normal lung fibroblasts hel 299 and mrc 5 cells while others use normal cell lines that don t belong to lung tissue. 16hbe 16 hbe 16hbeo 16 hbeo 16 hbe14o ecl ss 32011203. Through the removal and culturing of cancerous lung tissue in the explanted tumor of a 58 year old caucasian male.
Type i cells are large squamous cells whose thin cytoplasmic extensions cover 95 of the internal surface area. It is not present in the vocal cords of the larynx or the oropharynx and laryngopharynx where instead the epithelium is stratified squamous. Technical resources pulmonary alveolar epithelial cells paepic comprised of alveolar type i and type ii epithelial cells line more than 99 of the internal surface area of the lung. The cell lines used in this study are the beas 2b a human bronchial epithelial cell line wi 38 a diploid fibroblast cell line of human fetal lung tissue origin a549 and nci h1703 two human non small cell lung carcinoma nsclc cell lines all of which were collected in our laboratory.
Lung epithelial cells the investigation of the human lung and its diseases is an important issue for drug development processes although it is hampered by the availability of suitable in vitro models. Retains ion transport and other properties that are comparable to the native cells. 16hbe14o human bronchial epithelial cell line is widely used to model barrier function of the airway epithelium and to study respiratory ion transport as well as the function of cftr.
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