Lung Epithelial Cell Line A549

The cell line of a549 was cultured in atcc formulated modified f 12 k medium containing 10 fetal bovine serum 100 u ml penicillin and 100 u ml streptomycin sulfate.

Lung epithelial cell line a549. Cells from the human lung epithelial carcinoma cell line a549 atcc ccl 185 were maintained in dulbecco s eagle s medium supplemented with penicillin 0 6 μg ml 1 streptomycin 60 μg ml 1 glutamine 2 mm hepes buffer ph 7 4. Lieber m et al. A continuous tumor cell line from a human lung carcinoma with properties of type ii alveolar epithelial cells. A549 cell line origin.

Cross references nucleotide genbank. Through the removal and culturing of cancerous lung tissue in the explanted tumor of a 58 year old caucasian male. The a549 cell line was established in 1972 by d j. Cancer research the generation of epithelial models of thedistal lung for studies into airway function and disease and virology.

Human lung epithelial cells a549 which were bought from shanghai institutes for biological sciences. This adenocarcinomic cell line is categorized as a non small cell lung carcinoma nsclc which tends to. The a549 cell line was isolated in 1973 from a pulmonary adenocarcinoma. Cell culture nsclc cells a549 nci h23 nci h1299 and hcc827 and human normal lung epithelial cells beas 2b were bought from american type culture collection atcc manassas va usa.

Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen. In a study to. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. A549 cells were cultured in kaighn s modification of ham s f 12 f 12k medium atcc nci h23 nci h1299 and hcc827 cells were cultured in rpmi 1640.

Cell culture and seeding of a549. 20 mm and 10 fetal calf serum at 37 c. A549 a cell line model of nsclc used to evaluate the role of ccl22 and il 37 in epithelial mesenchymal transition 3 toxicology studies cellular toxicity of carbon based nanomaterials was tested in calu 1 and other lung cancer cell lines 4. Lung cancer and normal cell lines.

The a549 cell line is a suitable host for many human. Giard et al through an explant culture of adenocarcinomic lung tissue of a 58 year old caucasian male. A549 cells are hypotriploid alveolar basal epithelial cells. The cells are used as models for the study of lung cancer and the development of drug therapies against it.

Ccl 185 a549 lung carcinoma homo sapiens 58 years caucasian the cells are positive for keratin by. A549 cells are adenocarcinomic human alveolar basal epithelial cells and constitute a cell line that was first developed in 1972 by d. X54079 human mrna for heat shock protein hsp27.